Temporal Patterns of Gene Expression in
Murine Cutaneous Burn Wound Healing
Abstract:
The global changes in gene expression in injured murine skin were
characterized following a second-degree scald burn. Dorsal skin was
harvested from uninjured and from burned mice at two hours, and three
and 14 days following immersion in 65o C water for 45 seconds. Gene
expression was surveyed using an Affymetrix U74aV2 GeneChipä, and
patterns of gene expression were analyzed using hierarchical
clustering and supervised analysis. Burn injury produced significant
alterations in the expression of a number of genes, with the greatest
changes seen three and fourteen days after the scald burn. Using a
supervised analysis with a false discovery rate of 1% or 5%,
differences in the expression of 192 or 1116 genes, respectively,
discriminated among the unburned skin and the three time points after
the burn injury. Gene expression was primarily a transient and
time-dependent upregulation. The expression of only 24 of the 192
discriminating genes was downregulated after the burn injury. No gene
exhibited a sustained increase in expression over the entire 14 days
following the burn injury. Gene ontologies revealed an integrated
upregulation of inflammatory and protease genes at acute time
intervals, and a diminution of cytoskeletal and muscle contractile
genes at three or 14 days after the injury. Following a second degree
scald burn, global patterns of gene expression in the burn wound
change dramatically over several weeks in a time-dependent manner, and
these changes can be categorized based on the biological relevance of
the genes.
| Manuscript |
Physiol Genomics. 2004 Feb 13;16(3):341-8 |
| Figures |
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| Tables |
Table 1. 192 probe sets representing genes whose
expression discriminated (1% FDR) among the four experimental
conditions as determined by SAM |
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Table 2. 1116 probe sets representing genes whose
expression discriminated among the four experimental conditions as
determined by SAM (5% FDR) |
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Table 3. Ontology of genes whose expression was changed
from control, as listed by z-scores |
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| Raw Data |
Under Construction |
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