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Oncology
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RESEARCH INTERESTS: Dr. Kurenova is interested in adhesion-regulated signal transduction controlling mammalian cell growth and survival.
Her broad research goals are to understand the molecular events that regulate mammalian cell growth, differentiation, and motility, and to determine how these events go awry in cancer.
Dr. Kurenova's research efforts have focused upon signaling via FAK and serine/threonin kinase RIP. Three main aspects of FAK/RIP signaling are under investigation. First, experiments are being performed to elucidate the basic mechanisms by which FAK and RIP function. These include studies exploring interactions between FAK and RIP and other proteins. These analyses will provide insight into the mechanisms of regulation by upstream signals, engagement of components of downstream signaling pathways and targeting of FAK to discrete cellular locations. Second, the role of RIP and FAK interaction in cancer is being explored. Initial experiments are utilizing human breast epithelial and cancer cell lines and mouse embryonic fibroblasts deficient on FAK or RIP. FAK signaling has been perturbed by overexpression of dominant negative mutant of FAK and the consequences of altering FAK signaling assessed. Third,
Dr. Kurenova will perform experiments to examine the role of serine phosphorylation on FAK's signaling properties and adhesion-stimulated cellular responses and possible involvement of RIP in regulation of FAK activity through serine
phosphorylation. 1. Bass I. A., Kurenova E. V., and Mehedov, S. L. 1982 Cloning of the promoter located before the structural gene for the beta-subunit of the RNA polymerase of E. coli. Mol. Biol. (Russian) 16: 575-580. 2. Danilevskaya, O. N., Kurenova, E. V., Leibovitch, B. A., Shevelev, A. Yu., Bass, I. A., and Khesin, R. B. 1984 Telomeres and P-element of Drosophila melanogaster contain sequences that replicate autonomously in Saccharomyces cerevisiae. Mol. Gen. Genet. 197: 342-344. 3. Danilevskaya, O. N., Kurenova, E. V., and Kaverina, E. N. 1988 Localization of ARS in P element of D. melanogaster. Genet., Microbiol. & Virol. (Russian) 4: 1-44. 4. Leibovitch, B. A., Kurenova, E. V., and Danilevskaya, O. N. 1990 Variability of in situ hybridization of labelled probes with telomeric and some internal regions of Drosophila chromosomes. Cytology & Genetics (Russian) 24: 23-28. 5. Danilevskaya, O. N., Kurenova, E. V., Leibovitch, B. A., Bass, I. A., and Pavlova, M. N. 1990 The family of Drosophila genomes' repeated sequences having telomeric localization: properties and polymorphism. Genetics (Russian) 26: 474-484. 6. Kurenova, E. V., Leibovitch, B. A., Bass, I. A., Bebikhov, D. V., Pavlova, M. N., and Danilevskaya, O. N. 1990 Hoppel - the family of D. melanogaster mobile elements flanked by short inverted repeats and localized preferentialy within heterochromatic genomic regions. Genetics (Russian) 26: 1701-1712. 7. Danilevskaya, O. N., Kurenova, E. V., Pavlova, M. N., Bebikhov, D. V., Link, A. J., Koga, A., Vellek, A., and Hartl, D. L. 1991 He-T family sequences in Y chromosome of D. melanogaster share homology with the Y-linked Stellate genes. Chromosoma 100: 118- 124. 8. Danilevskaya, O. N., Petrov, D. A., Pavlova, M. N., Koga, A., Kurenova, E. V., and Hartl, D. L. 1992 A novel repetitive DNA element associated with telomeric sequences in D. melanogaster. Chromosoma 102: 32-40. 9. Danilevskaya, O. N., Lofsky, A., Kurenova, E.V., and Pardue, M.-L. 1993 The Y chromosome of Drosophila melanogaster contains a distinctive subclass of HeT-A-related repeats. Genetics 134: 531-543. 10. Kurenova, E., and Mason, J. M. 1997 Telomere functions. A review. Biochemistry (Mosc) 1997 Nov; 62(11):1242-53 11. Kurenova, E., Champion, L., Biessmann, H., and Mason, J. M. 1998 Directional gene silencing induced by a complex subtelomeric satellite from Drosophila. Chromosoma 107: 311-20 12. Mason, J. M., Haoudi, A., Konev, A.Y., Kurenova, E., Walter, M. F., Biessmann, H. 2000 Control of telomere elongation and telomeric silencing in Drosophila melanogaster. Genetica 109: 61-70. 13. Kurenova E., Xu L-H., Yang X., Baldwin A.S. Jr., Craven R.J., Hanks S.K., Liu Z-G., and Cance W.G. 2004 The Focal Adhesion Kinase Suppresses Apoptosis by Binding to the Death Domain of Receptor Interacting Protein. Mol Cell Biol. 24:4361-4371. 14. C. A. Garces, E. V. Kurenova, V. M. Golubovskaya, and W. G. Cance Vascular Endothelial Growth Factor Recptor-3 (VEGFR-3) and Focal Adhesion Kinase (FAK) bind and suppress apoptosis in breast cancer cells. (Submitted to Journal of Cancer Research, 2005).
1. Biessmann, H., Walter, M. F., Kurenova, E., and Mason, J. M. 1996 Retrotransposons at Drosophila telomeres and terminal chromosome deficiencies. Chromosomes Today, Vol. 12, 95-112.
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